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Worksheet: ISO 14644-1 Sampling Location Count and Minimum Sample Volume

A plug-and-play calculation worksheet for cleanroom classification: determining the number of sampling locations from cleanroom area, the minimum single-sample volume per location from the class concentration limit, and the sampling time, with the formulas, a worked example, and inspection notes.

Document type: Form

Read and copy the template below into your own quality system. It is a generic starting point for your own internal use, provided as is, with no warranty; see the Terms and License. Adopting it does not by itself create compliance.

This worksheet sets the two numbers that drive a cleanroom classification: how many locations to sample, and how much air to draw at each. It gives the formulas and the method; you look up the location count from the current standard and plug in your own class limit. The calculation mechanics here are physical formulas and conventions, not reproduced standard text; confirm every value against ISO 14644-1:2015 before you rely on it. A worked example follows. This content is educational and general.

Step 1: define the classification basis

FieldEntry
Room / area ID<<FILL>>
Target ISO class (state)<<FILL: e.g. ISO 5, at rest>>
Cleanroom area, m2<<FILL>>
Considered particle sizes<<FILL: e.g. 0.5 um; 5.0 um where required>>
Class concentration limit Cn,m for the most stringent considered size, particles/m3<<FILL: from the standard>>

Step 2: number of sampling locations

Under ISO 14644-1:2015 the minimum number of sampling locations is read from the standard’s Annex A lookup table by cleanroom area (the 2015 revision replaced the older square-root-of-area rule with a fixed table tied to a 95% upper confidence approach). Do not use the pre-2015 square-root shortcut.

FieldEntry
Area band from ISO 14644-1:2015 Table A.1<<FILL>>
Minimum number of locations, N<<FILL: read from the table>>
Locations laid out to represent the whole space, including process-critical points (over the filling zone, near returns, at work height)?Yes / No

Step 3: minimum single-sample volume per location

The minimum single-sample volume ensures you would expect to count at least 20 particles at the class limit for the most stringent considered size:

Vs = (20 / Cn,m) x 1,000 litres

where Cn,m is the class concentration limit (particles/m3) for that size. Apply two floors: never less than 2 litres, and never less than a one-minute sample.

FieldEntry
Cn,m used (most stringent considered size), particles/m3<<FILL>>
Vs = (20 / Cn,m) x 1000, litres<<FILL>>
Applied minimum (>= 2 L and >= 1 minute)<<FILL>>
Practical sample volume chosen, litres<<FILL: often 1,000 L / 1 m3, justified by the largest considered size and the need to resolve low counts>>
Basis for the practical volume<<FILL>>

Note: for ISO 5 at 0.5 um alone (Cn,m = 3,520) the formula yields only about 5.7 litres, so the common 1 m3 per point comes from the largest considered size (a lower concentration limit) and the practical need to resolve very low counts reliably, not from the 0.5 um limit.

Step 4: sampling time per location

FieldEntry
Counter flow rate, L/min<<FILL: e.g. 28.3 (1 cfm)>>
Time per location = volume / flow rate, minutes<<FILL>>
Total classification time (N x time per location), plus setup<<FILL>>

Step 5: sign-off

RoleNameSignatureDate
Prepared by<<FILL>>
QA reviewed<<FILL>>

References

ISO 14644-1:2015 (classification, location count and sample volume method). EU GMP Annex 1 (2022) for the grade-to-class mapping and where a 5.0 um figure applies.


Worked example

A 24 m2 Grade B background, classified at rest, target ISO 5 at 0.5 um.

FieldValue
Area24 m2
Locations N (from the 2015 Annex A lookup for that area band)6
Cn,m at 0.5 um for ISO 53,520 particles/m3
Vs = (20 / 3,520) x 1,000about 5.7 litres
Applied minimum5.7 L exceeds the 2 L and 1-minute floors
Practical volume chosen1,000 L (1 m3) per location, to resolve low 5.0 um counts reliably
Counter flow28.3 L/min
Time per locationabout 35 minutes
Total (6 locations)about 3.5 hours plus setup

The example shows why the 0.5 um formula alone (5.7 L) is not what drives the sample size; the practical 1 m3 comes from the largest considered particle size and the need to count reliably at very low concentrations.

Common inspection findings this worksheet prevents

  • Using the pre-2015 square-root-of-area rule for the location count.
  • A sample volume too small to detect particles at the class limit for the most stringent size.
  • Locations that do not represent the room or miss the process-critical points.
  • No record of how the sample volume was justified.

How to adapt this worksheet

  1. Read the location count from the current ISO 14644-1 Annex A table for your exact area.
  2. Use the class limit for your most stringent considered size in the Vs formula.
  3. Record the practical volume and the basis, so the choice is defensible.
  4. Reuse the location layout across classification, EM, and requalification for consistency.
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